ABSTRACT
OBJECTIVE@#To study the relationship of the expressions of p53 and mdm2 in leukoplakia cancer.@*METHODS@#RT-PCR was used to detect the mRNA of p53, mdm2 in patients with leukoplakia cancer. The frequencies of p53, mdm2 in peripheral blood were detected by flow cytometric analysis.@*RESULTS@#The expression of p53mRNA in normal oral mucosa, simple oral leukoplakia, no-simple oral leukoplakia and leukoplakia cancer were 7.7%, 27.3%,33.3%, 56.8%, respectively. The frequencies of p53 in normal oral mucosa, simple oral leukoplakia, no-simple oral leukoplakia and leukoplakia cancer were (0.3±0.1)%, (1.6±0.9)%, (1.9±1.1)%, (3.4±1.8)%. The expression of mdm2 mRNA in normal oral mucosa, simple oral leukoplakia, no-simple oral leukoplakia and leukoplakia cancer were 0.0%, 6.8%, 11.1%, 37.8%, respectively. The frequencies of mdm2 in normal oral mucosa, simple oral leukoplakia, no-simple oral leukoplakia and leukoplakia cancer were (0.1±0.1)%, (0.8±0.6)%, (1.2±0.8)%, (1.2±0.8)%. There was a positively correlation between p53 mRNA and mdm2 mRNA.@*CONCLUSIONS@#The positive rate of p53 and mdm2 cells in the peripheral blood increases in patients with leukoplakia cancer tissue and has positive correlation with the severity of leukoplakia cancer.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Gene Expression Regulation, Neoplastic , Leukoplakia , Genetics , Metabolism , Proto-Oncogene Proteins c-mdm2 , Genetics , Metabolism , Tumor Suppressor Protein p53 , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the difference between the results of using two standards(CPI and No.5 sharp probes) in the diagnosis of clinical dental caries during epidemiological survey.</p><p><b>METHODS</b>The CPI and No. 5 sharp probes were used respectively for the diagnosis of clinical dental caries in the same group of children aged 12 by the two examiners. This group of children were reexamined after 14 days to compare the consistency and the rate of coincidence of the location of teeth and caries average and the prevalence rate of dental caries diagnosed by the two kinds of probes.</p><p><b>RESULTS</b>The difference between the location of teeth with caries examined by the two different standards respectively by the same examiner was not statistically significant. The difference between caries average and the prevalence rate of dental caries examined with the two different standards respectively by the same examiner was also not statistically significant. The difference between the outcomes of the crossover comparison by the two examiners showed no statistical significance.</p><p><b>CONCLUSIONS</b>There was no significant difference between the two diagnostic standards for caries. The outcomes of the diagnosis based on both standards shows a similarity. CPI could replace No. 5 sharp probes due to its convenience in large scale of oral health survey.</p>
Subject(s)
Child , Female , Humans , Male , China , Epidemiology , DMF Index , Dental Caries , Diagnosis , Epidemiology , PrevalenceABSTRACT
<p><b>OBJECTIVE</b>To survey the prevalence of dental caries and the needs for dental treatment in Anhui province.</p><p><b>METHODS</b>The survey of prevalence of dental caries in group aged 12 and 15 years in Anhui province was conducted using the non-proportional, stratified, cluster sampling method.</p><p><b>RESULTS</b>DMFT in 12-year and 15-year group was 0.58 and 0.95 respectively, and permanent tooth caries prevalence rate was 30.78% and 39.78% respectively, lower than that of same age of other 11 provinces in mainland China (P < 0.01). The constituent ratio of DMFT was 90.51% (D), 2.48% (M), 7.01% (F) in 12-year group and 86.78% (D), 4.30% (M), 8.92% (F) in 15-year group.</p><p><b>CONCLUSIONS</b>When we controlled dental caries in 19.98% 12-years crowd and 22.8% 15-years crowd of Anhui province, we will had controlled the occurrence of 80% caries too in group aged 12 and 15 years.</p>